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CHF122SC Versatile Micro to Prep Fraction Collectors

CHF122SC Versatile Micro to Prep Fraction Collectors

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Description

From open column to HPLC liquid chromatography and general liquid samplings, the Super Fraction Collector, model CHF122SC has a wide range of uses.
The CHF122SC is a fraction collector with increased reliability, employs an X-Y movement mechanism based on long years of technological accumulation, a new vertical movement apparatus and a large display screen for easier use than ever. The CHF122SC holds up to 120 vessels, and can handle a variety of vessel diameters and lengths. With its numerous fraction functions, the CHF122SC boasts a wide range of applications.

User friendly operation

  • Features five separate operating modes (simple, peak, window, manual and sampling) to suit your application needs.
  • Adjustable sizes of collection vessels give user room for versatility. The standard test tube rack holds 120 tubes with a diameter of 12-15mm. Remove the standard rack holder to use with tubes up to 18.1mm.
  • A shifting-type nozzle lets you deliver liquids accurately and quickly into a specified tube position.
  • Built in wait drain allows discharge of buffer or waste solution to prevent contamination in test tubes.
  • Sturdy and reliable construction of polypropylene and ABS plastic protects from water droplets and is flame retardant for long term use and protection.
  • Display features 9 operation keys with large characters to allow user ease of programming. Graphical display can verify fraction collection results, displaying up to 500 events.

Simple mode: collect by time, drops, volume or signal. Peak mode: specify up to a maximum of 10 peaks for identification. Peak determination may be made using a combination of absolute value and slope angle.

Window Mode: a maximum of 16 windows (time ranges) may be set so that peaks are identified and fraction collection occurs only within each window. By setting the monitor time, non-peaks may also be collected in test tubes.

Manual mode: collect fractions while watching the chromatography signals displayed on the screen. A fraction may be reviewed under playback mode.

Sampling mode: enables the user to take samples from the effluent at specified time intervals during a set period and collect repeat samples into the same vessel.

Loaded with Unique Collection Functions
Simple mode: Collect by time, drops, volume or signal.
Peak Mode: Specify up to a maximum of 10 peaks for identification. Peak determination may be made using a combination of absolute value and slope angle.

Example of peak absolute value setting Set the percent value using an input signal full scale of 100%. Peaks above the set absolute value are identified.

Example of slope angle setting. As shown in the graph, because of base line drift, only three peaks are identified at 1, 2/3 and 4/5/6/7/8 by absolute value setting. However, by using a slope setting, all 8 peaks are identified.

Window mode: A maximum of 16 windows (time ranges) may be set so that peaks are identified and fraction collection occurs only within each window. By setting the monitor time, non-peaks may also be collected in the test tubes.

Window mode setting, example 1
Window 1 is set between 2min00sec and 2min32sec at request time of 12sec.
Window 2 is set between 3min32sec and 5min00sec at 8 sec request time.

  • Main settings
    Wait time: [40 sec]
    Non-window monitor time: [0 sec]
  • Window settings
    01: Start [2min00sec] – End [2min32sec]Request time [12 sec]
    02: Start [3min32sec] – End [5min00sec]Request time [8 sec]
  • Peak parameters
    Absolute value: [0%], Slope angle: [0mv/min]
    Response: [*HIGH], Delay time: [0 sec]

Window mode setting, example 2 (peak identification)
Peaks within the windows are identified. Fraction collection is set at 12 seconds during peaks for window 1. For non-peaks, the monitor time interval is 8 seconds.

  • Main settings
    Wait time: [40 sec]
    Non-window monitor time: [0 sec]
  • Window settings
    01: Start [2min00sec] – End [2min32sec]
    Request time [12 sec]
    Monitor [08 sec]
    02: Start [3min32sec] – End [5min00sec]
    Request time [8 sec]
    Monitor [24 sec]
  • Peak parameters
    Absolute value: [40%]
    Slope angle: [20 mV/min]
    Response: [*HIGH]
    Delay time: [0 sec]

Manual mode: Collect fractions while watching the chromatography signals displayed on the screen. A fraction may be reviewed under playback mode.

Sampling mode: Collect fractions for a set interval during a set period. Multiple collections may be set per test tube. In the setting example shown on the right, wait time is 5 minutes, each test tube has 2 repeats of 5 minutes intervals every 15 minutes.