Loading... Please wait...

Linear Dichroism Couettes

Price: Contact us by e-mail: inbox@kromatek.co.uk


linear-dichroism-couettes.jpg 

Ultra Low Volume Linear Dichroism Couette Flow Cell

Typical Applications include Membrane protein structure Protein fibre structure DNA and DNA binding molecules Polymer Structure

Low volume (40 μL)
High alignment 500 μm
Reuseable sample holder
Simple installation

Long molecules such as fibrous proteins are particularly difficult to characterise structurally. The use of a Micro Volume Couette Flow Linear Dichroism (LD) cell with a sample volume of only 40 μL is ideally suited for use with biological samples.

Couette flow orientation is created by sandwiching the sample between two cylinders, one static whilst the other rotates. This has proved to be the optimal technique employed for Linear Dichroism experiments in many laboratories around the world.

linear-dichroism-couettes-3.jpg
linear-dichroism-couettes-4.jpg 

Dioptica Capillary Micro Volume Couette Flow LD Cells utilise extruded quartz rods and capillaries positioned in some cases between focusing and collecting lenses and is available in non-thermostatted, electrically heated and Peltier† thermostatted versions.

Dioptica manufacture the instrument, which was inspired and developed by the research requirements of Professor Alison Rodger at the University of Warwick.


linear-dichroism-couettes-5.jpg 

Dioptica LD Cell is currently supplied for use with JASCO J-Series Circular Dichroism Spectropolarimeters.

Each LD Cell is supplied with a base plate to fit either the standard or large sample compartment. In addition, a spare light-tight cover plate is provided to enable the electrical connections of the controller to connect to the LD Cell in the sample compartment of the spectropolarimeter.

 linear-dichroism-couettes-8.jpg   linear-dichroism-couettes-9.jpg

The electrically heated version is designed to work isothermally. The Peltier version requires that the user has an existing JASCO Peltier controller which enables the unit to be programmed from JASCO Spectra Manager™ software allowing both isothermal and temperature ramping experiments.

Ordering Information and Specifications

DIOP-0002 Dioptica Ultra Low Volume Flow Linear Dichroism
Accessory (non-thermostatted)
Sample container composition: Laboratory grade stainless steel
Optical components: Quartz
Wavelength range: 185-800 nm
Pathlength: 500 μm
Capilliary: Outer diameter 5 mm inner 2.9 mm central rod 2.4 mm typically
Optimal sample volume: 40 microlitres depending upon capillary
Motor drive: 6V direct drive via flexible coupling
Speed range: 0-7000 rpm
For JASCO J-Series CD Spectrometers supplied with 10 capillaries and 3 rods with nylon holder


DIOP-0003 Dioptica Ultra Low Volume Flow Linear Dichroism
Accessory with electrically heated thermostatting
Temperature Range: Electrically heated ambient to 90°C
Sample container Composition: Laboratory Grade Stainless Steel
Optical components: Quartz
Wavelength range: 185-800 nm
Pathlength: 500 μm
Capilliary: Outer diameter 5 mm inner 2.9 mm central rod 2.4 mm typically
Optimal sample volume: 40 microlitres depending upon capillary
Motor drive: 6V direct drive via flexible coupling
Speed range: 0-7000 rpm
For JASCO J-Series CD Spectrometers supplied with 10 capillaries and 3 rods with nylon holder

DIOP-0004 Dioptica Ultra Low Volume Flow Linear Dichroism
Accessory with Peltier thermostatting capability†
Temperature Range: Ambient to 90 °C
Sample container Composition: Laboratory Grade Stainless Steel
Optical components: Quartz
Wavelength range: 185-800 nm
Pathlength: 500 μm
Capilliary: Outer diameter 5 mm inner 2.9 mm central rod 2.4 mm typically
Optimal sample volume: 40 microlitres depending upon capillary
Motor drive: 6V direct drive via flexible coupling
Speed range: 0-7000 rpm
For JASCO J-Series CD Spectrometers supplied with 10 capillaries and 3 rods with nylon holder

linear-dichroism-couettes-fig1.jpg

Schematic diagram of an LD Couette cell:

(a) stationary inner and outer cylinders therefore no sample orientation and no net LD signal
(b) rotating inner and stationary outer cylinder resulting in sample orientation and thus an LD signal.

linear-dichroism-couettes-fig2.jpg 

Download PDF Brochure

Email Sign-up to the Newsletter

Sign-up for the latest products, promotions...