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Sample Syringe Filters

Guide to selecting the right syringe filters

1. Choose membrane type

  • Nylon - Broad solvent compatibility, Aqueous and organic materials.
  • PTFE - Ideal for HPLC, UHPLC sample preparation and excellent solvent resistance
  • PES (Polyethersulfone) - Highest flow rates, ideal for Ion chromatography, low protein binding for biological samples.
  • PVDF (Polyvinylidene) - Broad chemical compatibility, fast flow, low protein binding for biological samples. Low UV absorbing extractables for HPLC & UHPLC.

Aqueous Samples – Use syringe filters with an affinity for water (hydrophilic) Nylon or PES  Syringe filters

Non polar samples – Use syringe filters which are more hydrophobic such as PTFE, PTFE is chemically resistant and often used with HPLC solvents such as acetonitrile/water and aggressive organic solvents

Protein Samples –Use PVDF or PES, low protein binding membrane syringe filters and therefore suitable for life science applications 

Inorganic Samples – Use PES membrane syringe filters for applications like ion chromatography.

2. Choose Filter size 

 0-10ml Sample Volume use 13mm Syringe Filters ; 10-100ml Sample Volume use 25mm Syringe Filters; 10-250ml Sample Volume use 33mm Syringe Filters and for fastest flow rates 

3. For HPLC 

 Use 0.2u syringe filters for sub 2.7u columns (UHPLC )and use 0.45 u filters for normal particle size columns


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